Characterization and siderophores production of Rhizobium spp. isolated from wild legumes

Abstract

Legume plants are very significant not only ecologically but also agriculturally because they are responsible for major change of nitrogen from atmospheric N2 to ammonia. In this study, total 56 isolates of Rhizobium spp. which were previously isolated from wild legumes plant (Melilotus officinalis, Medicago sativa and Vicia cracca) existing in the central and the disricts of Kırşehir province (Kaman, Mucur, Akpınar, Akçakent, Çiçekdağı, Boztepe). In order to characterize the isolates; YMA containing Bromothymol blue, Congo red, Gram stain reaction , movement, catalase and oxidase tests were evaluated. In addition, isolates of Rhizobium spp. (wild type) were screened for their ability to produce siderophores and it was determined that 50 of 56 isolates in total can produce siderophore.