Heterologous Expression and Molecular Cloning from Williamsia Marianensis

Abstract

The majority of therapy methods include downsides and limits. As a result, many researchers are focused on developing effective remedies. Therapeutic peptides, like proteins and antibodies, are a potential class of medications that have a number of advantages over traditional pharmaceuticals. Williamson marianensis-produced cholesterol oxidase has been demonstrated to have medicinal value. Using PCR and primers specific to an expression vector (pET28b), we were able to clone the cholesterol oxidase gene and express it in E. coli (BL-21/DE3) Rosetta following identification with IPTG. Genscript Corporation in the United States sequenced gyncholestrol oxidase (500 bp) to create a cox sequence, which was then submitted for synthesis. pET 28a(+) cox William showed a twofold restriction digestion pattern. The pattern was made up of two strands: one was a carrier plasmid (4200 bp) and the other was a 2800 base pair strand that contained the cholesterol oxidase gene. The cholesterol oxidase gene was successfully cloned and expressed as a consequence. Williamson marianensis-derived cholesterol oxidase will be exploited in future medicinal re