Unlocking Rotavirus Defense: Predicting B Cell Epitopes Targets for Human Rotavirus Type A Vaccines
DOI:
https://doi.org/10.22399/ijcesen.2287Keywords:
Rotavirus, vaccine design, in-silico methods, epitope prediction, immunoinformatics, immunogenicity predictionAbstract
Rotavirus, an exceptionally contagious viral agent, holds the distinction of being the primary causal factor behind acute infectious diarrhea in the pediatric demographic under the age of five, thus constituting a profound menace that transcends geographical boundaries. Despite commendable strides in the realm of vaccine development, the terrain of vaccine design and efficacy continues to be rife with challenges. Within the ambit of this investigation, we undertake an exploration of an in-silico avenue for the formulation of rotavirus vaccines, thereby embarking upon a probing analysis of prospective vaccine candidates. The protein sequence of Human Rotavirus Type A was obtained from the UniProt database, and BepiPred 3.0 and ABCpred tools were employed for epitope predictions. Additionally, the secondary structure of RNA molecules for the Human Rotavirus Type A vaccine design was analyzed by utilizing the predictive capabilities of the RNAfold Server. The Class I MHC Immunogenicity and SVMTriP programs were used to predict immunogenicity, while Vaxijen 2.0 was employed to forecast immune properties. According to the results obtained from our study, the CGATGTTGTTGATGGT sequence exhibits epitope potential. This study has provided insights into the analysis of the secondary structure of RNA molecules for the design of Human Rotavirus Type A vaccines, enabling the identification of potential immunogenic regions that could serve as targets for vaccine candidates.
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